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NANOBASE PRODUCT PORTAL

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Raman Spectroscopy Instruments

TRPL Measuring Instruments

XperRF is a two-in-one instrument with a focus on enhanced material analysis. The instrument has a Raman spectroscopy module and a TRPL measurement module, both of which offer useful techniques to determine characteristics of a material. It's rather easy to note that this instrument is a combination of XperRAM S Series and Xper-FLIM.

With XperRF, you can enjoy all the high efficiency features of XperRAM S Series which includes high performance spectrometer and detector plus a wide and fast scanning scanner module. For a TRPL measurement module, we collaborate with PicoQuant, a renowned company from Berlin, Germany, to maximize the measuring
quality.

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Product No. XPERRF

  • XperRF is a.k.a. XperRAM Ultimate and XPERAM-RF

  • The fiber coupling port is an optional part.

What XperRF can do for you

  • Raman spectrum acquisition

  • Raman map image acquisition

  • Photoluminescence (PL) data acquisition

  • Electroluminescence (EL) data acquisition

  • Time-Resolved Photoluminescence (TRPL) data acquisition

  • There is a broad range of applications where you can utilize data of Raman, TRPL, PL, and EL - from biology/medical research to solar cell efficiency analysis. If you would like to consult about how these data can make a contribution to your research, we are just one message away! Please contact us now here.

 

TRPL? TCSPC? FLIM?

  • Time-Resolved Photoluminescence (TRPL) is a general term while Time-Correlated Single Photon Counting (TCSPC) or Fluorescence Lifetime Imaging (FLIm) are special techniques to achieve TRPL.

  • Fluorescence lifetime imaging microscopy or FLIM is an imaging instrument type for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample.

  • Fluorescence lifetime imaging yields images with the intensity of each pixel determined by τ, which allows researchers to view contrast between materials with different fluorescence decay rates, and also produces images which show changes in other decay pathways.

  • Fluorescence lifetimes can be determined in the time domain by using a pulsed source. When a population of fluorophores is excited by an ultrashort or delta pulse of light, the time-resolved fluorescence will decay exponentially.

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  • Time-correlated single-photon counting (TCSPC) is usually employed as a measurement method because it compensates for variations in source intensity and single photon pulse amplitudes. More specifically, TCSPC records times at which individual photons are usually detected by a single photon avalanche photo diode (SPAD) with respect to the excitation laser pulse.

  • The recordings are repeated for multiple laser pulses and after enough recorded events, researchers are able to build a histogram of the number of events across all of these recorded time points. This histogram can then be fit to an exponential function that contains the exponential lifetime decay function of interest, and the lifetime parameter can accordingly be extracted.
     

Raman?

  • Raman is a light scattering technique. Raman analyzes a small amount of light (laser) scattered at different wavelengths (or colors), which depend on the chemical structure of the analyte.

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  • By analyzing Raman spectra, we can identify what kind of material it is, so we simply call a Raman spectrum (Raman data realized in a graph format) as a fingerprint of a material. Analysis of Raman images which correspond with Raman spectra, is also in-demand Raman analysis technique.

  • TRPL data and Raman data show correlation. See the below image for data comparison between the two different techniques. The below data of single oral epithelial cell was taken from XperRF.

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Why XperRF can step up your TRPL game

Powerful TCSPC module supplier

Reliable and ultra fast TCSPC module

  • With an optional dual channel time tagging electronics, you can achieve a just 10-second TRPL mapping time for 50 µm x 50 µm of a scanning area. Check out the below video clip.

Why XperRF can step up your Raman game

High Raman peak efficiency = Precise Raman data

  • Not like most Raman manufacturers, we have adopted transmission grating in a Raman spectrometer to boost efficiency to over 90%.

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  • The spectrometer for XperRF (XPE200) coupled with an OEM CCD by ANDOR is also designed for a high efficiency.

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Wider and faster laser scanning

  • Our realization of wide, fast laser scanning capability using a single Galvo mirror maximizes even our scanning efficiency and helps you see a bigger picture of your Raman data.

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  • Why laser scanning? It reduces production cost yet maintains excellent performance.

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  • Still not convinced? Check out the below video for the actual mapping process of XperRF.

High signal to noise ratio = Great Raman spectral resolution

  • For a better spectral resolution of Raman data produced by XperRAM S Series (the Raman module of XperRF), the system boasts a high signal to noise ratio via a transmission grating and the smallest possible number of optics.

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System flexibility

  • XperRF offers a variety of Raman system options to meet your research needs.

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What XperRF is made of

  • Microscope

- Upright microscope or inverted microscope

  • System platform

- Main body of the instrument

  • Objectives

- Default : 40X
- Optional : 10X, 20X, 50X, 100X, Long WD 40X

  • Spectrometer (XPE 200)

- f/5 input aperture ratio
- 200 mm focal length
- Grating options : 300 lpmm, 600 lpmm, 1200 lpmm, 1800 lpmm, 2400 lpmm

  • Laser scanning module

- If scanning not required, replaced by point measuring module

  • Detector

- 2000 x 256 active pixels
- 15 
µm x 15 
µm pixel size
- Back-illuminated, deep-deletion with anti-fringing

  • Raman lasers and filter sets

- Optional : 405 nm, 532 nm, LF 532 nm, 633 nm, 785 nm, 1064 nm (via customization)

  • TCSPC module

- RF platform (Raman-TRPL switch box)
- Single photon avalanche detector (SPAD)
- Time tagging electronics
- 405 nm Picosecond pulsed diode laser and driver
- 405 nm PL filter for TCSPC 

  • NanoSpectrum software suite

- Spectrum data export format : .txt., .csv
- Mapping data export format : .spm, .csv
- Optional : Raman library and data identification software (to be launched soon)

Downloads

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