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- A beam of light, usually ultraviolet light, excites the electrons in molecules of certain compounds and causes them to emit light of a lower energy. By analyzing the different frequencies of light emitted in fluorescent Spectroscopy, along with their relative intensities, the structure of the different vibrational levels can be determined.
Pic.1 Raman Effect
Because most of molecules exist at ground state, as you
see from the above graph, by tracing the intensity of
Stokes band, increase and decrease of molecules can be
known.
Consequently, a location of Stokes band shows energy of
vibration mode and an intensity of band shows quantity of
molecules. Finally, we can examine the properties and
structure of the samples, obtaining Raman Spectrum.
